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KMID : 1149220000080010147
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Korean Journal of Oriental Medical Prescription
2000 Volume.8 No. 1 p.147 ~ p.167
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Protection by Sunghyangchungisan against Oxidative Endothelial Cell Injury
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Lee Dong-Eon
Kim Young-Gyun
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Abstract
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Reactive oxygen species (ROS) play an important role in the pathogenesis of a variety of life threatening conditions such as atherosclerosis, myocardial infarction and cerebral stroke. In this study, the effect of Sunghyangchungisan (SHCS) as a cytoproctant against ROS-induced cell injury was studied by investigating its effect on H₂O₂-induced cell injury in cultured endothelial cells derived from the human umbilical vein. SHCS effectively proteced the cells against H₂O₂-induced injury determined by trypan blue exclusion ability and lactate dehydrogenase (LDH) release. The effect of SHCS was concentration-dependent and the concentrations to inhibit by 50% the cell death and LDH release were and , respectively. In addition, SHCS effectively protected the cells against t-butylhydroperoside- and menadione-Induced injury as well. SHCS inhibited lipid peroxidation determined by malondialdehyde production. SHCS exerted as an effective scavenger of ROS produced by exposing the cells to H₂O₂ The activities of the intracellular ROS scavenging enzymes such as superoxide dismutase, catalase and glutathione peroxidase were not Influenced by SHCS.These results indicate that SHCS might exert as an effective cytoprotectant against ROS-induced cell injury. Further intensive studies would provide us insights into mechanisms of the pharmacological actions of SHCS.
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KEYWORD
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